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Goudanavar, P. S.
- Isolation and Characterization of Antibiotic Production from Soil Isolates by Fermentation
Authors
1 KLE College of Pharmacy, Nipani, Karnataka, IN
2 KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka, IN
3 KLES’s College of Pharmaceutical Sciences, Nipani, Karnataka, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 2, No 1 (2010), Pagination: 32-36Abstract
In screening of new antibiotics, several actinomycetes were isolated from soil samples. Crowded plate technique was used for the isolation of actinomycetes. The morphological and cultural characterization of A-4 strain was performed. In medium formulation study for A-4 and A-4 mutant, various carbon and nitrogen sources were tested for maximum antibiotic production using zone of inhibition and packed cell volume (%) as parameters. Various fermentation conditions like pH, temperature and DO2 were also optimized for the maximal production of antibiotic from both A-4 and A-4 mutant. All medium formulation as well as bioprocess parameters for A-4 and A-4 mutant strains was compared. Some actinomycetes strains, showed promising antimicrobial scores against different strains of bacteria and fungi. From the six strains selected, one strain designated as A-4 showed maximum antimicrobial property against gram positive and gram negative strains as well as various fungi. Morphological and cultural studies showed that A-4 is belongs to actinomycete genus.. The strain A-4 and A-4 mutant was found to be having better antimicrobial activity in comparison with other soil isolates of actinomycetes.
Keywords
Actinomycete, Antibiotic, Crowded Plate Technique, Zone of Inhibition, Fermentation.
- Characterization and Evaluation of Glibenclamide Transmucosal Drug Delivery System
Authors
1 K.L.E’s College of Pharmacy, Nipani –591237, Dist: Belgaum, Karnataka, IN
2 K.L.E’s College of Pharmacy, Nipani, Karnataka, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 2, No 1 (2010), Pagination: 44-46Abstract
In an attempt to develop mucoadhesive buccal drug delivery system, buccal tablets of Glibenclamide were prepared using polymers such as carbopol-934, Hydroxypropylmethyl cellulose HPMCK4M, and sodium carboxy methyl cellulose (Sod. CMC) in various proportions and combinations. The tablets were evaluated for different physicochemical parameters like weight variation, friability, hardness, drug content, water absorption studies, bioadhesive performance, release characteristics and surface pH. Tablets containing carbopol-934 and sodium CMC showed a maximum in vitro release of 82.27%. The formulations were subjected to graphical treatments according to Higuchi's equation and Peppa's equation. The best formulation F1 confirmed that the release mechanism is by diffusion, the rate of release following first order kinetic model.